Non-alcoholic fatty liver disease (NAFLD) is a major cause of chronic liver disease globally. Epidemiological studies suggest prevalence of NAFLD in around 9% to 32% of general population in India. A variety of studies have suggested that there is an apparent mild elevation in levels of AST and ALT enzymes which may serve as markers for NAFLD. Therefore we carried out the present study to find out the association between laboratory data and NAFLD and to evaluate and confirm non-invasively the usefulness of serum biochemistry for the diagnosis of NAFLD.