The distribution of various bacterial isolates causing infectious diseases was evaluated in Karpagam Medical College Hospital, Othakkalmandapam, Coimbatore district, Tamilnadu. A total of 578 samples which included urine, pus, sputum, blood, eye swab, throat swab, ear swab, nasal swab, ascitic fluid, pleural fluid and C.S.F were analyzed. Out of total 578 samples analyzed in bacteriology laboratory, 216 were culture positive. The antibiotic susceptibility test of the bacterial isolate was performed by Kirby-Bauer disk diffusion method. Majority of the bacterial isolates showed wide resistance to the antimicrobials employed. High rate of multiple antibiotic resistances was observed in isolates from I.C.U and Surgery Departments.