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Rp-hplc method development and validation for the simultaneous estimation of prazocin and polythiazide in bulk and tablet dosage form

Author: 
Ramakrishna, B., Supriya, P., Venkatalakshmi, V. and Naidu, N.V.S.
Subject Area: 
Life Sciences
Abstract: 

A simple, Accurate, precise method was developed for the simultaneous estimation of the Prazocin and Polythiazide in Tablet dosage form. Chromatogram was run through Std Discovery C18 150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 270.0 nm. Retention time of Prazocin and Polythiazide were found to be 2.316 min and 3.176. %RSD of the Prazocin and Polythiazide were found to be 0.5 and 0.7 respectively. %Recovery was obtained as 100.49% and 100.40% for Prazocin and Polythiazide respectively. LOD, LOQ values obtained from regression equations of Prazocin and Polythiazide were 0.079, 0.240 and 0.03, 0.08 respectively. Regression equation of Prazocin is y = 50050.x + 7773 and of Polythiazide was y = 95434.x + 6175. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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