In the present study, sero-prevalence of anaplasma sp. in sheep, Ovisaries (L) was done from January-May, 2012 in Peshawar, Pakistan. The information concerning anaplasmosis in sheep is scare. For this purpose, 376 serum samples were obtained randomly from4 different breeds of sheep, from different areas of Peshawar, and an indirect ELISA using recombinant MSP-5 as antigen of Anaplasmamarginale (T), was performed. Totally, 92/376 (24.47%) of the overall sheep sera were positive for antibodies against A. marginale. In 4 breeds of sheep, (i.e. Balkhai, Watanai, Punjabai and Turkai) Turkai were found highly infected i.e. 27/376 (7.20%). This is the first record of A. marginal infection in Sheep in Peshawar, Pakistan, which is very high. This research should be useful in epidemiological applications.
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