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In Silico Analysis Of Vaccine Target Against Multidrug Resistant Staphylococcus Aureus

Author: 
Shuchi Kaushik , Archana Shrivastav, and Sudhir Kumar Jain
Subject Area: 
Life Sciences
Abstract: 

Staphylococcus aureus (S. aureus) is a common bacterium that lives on the skin and in some people’s noses. S. aureus can cause a range of mild to severe infections. Excessive use of antibiotics has led to drug-resistant strains of S. aureus (MRSA). The ancient practice of vaccine designing was being evaluated in the present study for the in silico development of preventive measure against multidrug resistant Staphylococcus aureus infections. Multiple sequence alignment revealed various conserved regions in mecA protein. We predicted one such type of multi-epitope peptide which was having very good potential to induce B cell response and a very good candidate for binding to MHC II molecule. Structure prediction of this sequence by PSIPRED revealed that 22 helix are present in this sequence. The identified peptide can be a suitable target for induction of both TH cell and B cell. This peptide was designed from conserved regions of mecA, so it can be a preventive measure for MRSA infections after suitable experimental analysis. This data can be very helpful for generating antigenic candidate by wet lab researchers.

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