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Stability indicating rp- hplc method for estimation of nisoldipine in bulk and tablet dosage form

Author: 
Vishnu Vardhan, D., Venkateshwar Reddy, K. and Srinivas Nayak, A.
Subject Area: 
Health Sciences
Abstract: 

A simple reverse phase HPLC method was developed for the estimation of Nisoldipine in bulk and tablet dosage form. Chromatography was performed by isocratic elution on a Stainless steel Thermo scientific C18 column with dimensions 4.6 x 250mm, packed with octadecylsilane bonded to porous silica (C18) with particle size 5 micron acetonitrile and water in the ratio of 80:20 % v/v is used as mobile phase. The flow rate is 1.0 ml/ min and effluent is monitored at 240 nm. Nisoldipine was eluted at a retention time of 4.4 minutes. The standard curve of Nisoldipine was linear over a working range of 0.3– 8 µg/ml and gave an average correlation coefficient of 0.999. The limit of quantitation (LOQ) of the drug is 0.1 µg/ ml. Recovery studies were carried out by standard addition method and the recoveries are found satisfactory within the range of 99.24 to 99.6 %.The method is precise with % RSD below 2. The method is validated in terms of robustness and forced degradation studies were carried out.

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