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Staining of histological sections from liver tissues using curcuma longa

Author:

Abd-Alhafeez Osman Ibnouf, Esam M. A. Suliman, Nibras Abdelmajed Babeker, Abrar Khalid Abdalmjeed and Esmail Seddiq Esmail Saed

Subject Area:

Health Sciences

Abstract:

Background and Objectives: naturalplantdyeshave gained worldwide interest and give encouraging tissue staining results when tested. This study aimed to assess the quality of staining of hepatictissues by Hematoxylin and curcuma longa solutions compared to Hematoxylin and Eosin routine stain. Methods: tissue biopsies were obtained from a liver of a healthy rabbit and stained using Curcuma Longa solution instead of eosin in the different Hematoxylin-eosin protocols. Results:The best results were obtained with the time duration 10 – 20 min when using Weiger's Hematoxylin. Interpretation and Conclusions:Curcuma longa extract is a promising histological dye that effectively can replace Eosin stain in the Hematoxylin &Eosin staining protocol.

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