Standardization of one-step single tube rt-pcr for rapid detection of all circulating strains of dengue viruses

Dengue is a viral disease which is spread by a mosquito vector and is prevalent in tropical and subtropical regions. Dengue poses serious health threat to a major portion of population therefore rapid and specific tests are needed to combat the situation. Thus, one-step reverse transcription polymerase chain reaction (RT-PCR) was standardized to detect all the four serotypes of dengue viruses i.e. DENV1 to DENV4. This study was performed on standard strains of dengue viruses. RNA was extracted from these strains and was used to optimize one step RT-PCR. This assay was able to detect all the four serotypes of dengue virus.