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Studies on anti-bacterial properties of sidarhombifolia plant

Author: 
Nkemaja Dydimus Efeze, Nantia Akono, E., Azeteh Ignatius N., Njeugna Ebenezer, Murugesh Babu, K. and Zipo Rodulf
Subject Area: 
Life Sciences
Abstract: 

The antibacterial properties of fabrics are being considered to be an important and inevitable parameter for garment especially those that are in direct contact with the body. The main aim of this research is to investigate the antibacterial activity of ethanol extract of Sidarhombifolia against bacterial strain Staphylococcus aureasand Streptococcus pyogene and evaluate the phytochemical screening of the extract. Extract from 3 different parts of sidarhombifolia was extracted using ethanol as solvent. The bacterial inoculum was prepared to activate the bacteria growth and the activated bacterial were cultured using well diffusion method and incubated for 24 hours. The quantities of extract yielded from the leaves, stems and retted fibre were 2.13%, 1.8% and 1.0% respectively. The extracts were tested on Staphylococcus aureus and Streptococcus pyogenes at 4 different concentrations using wells diffusion method and the diameter of inhibition was measured and recorded. From the value of diameter of inhibition obtained, it was noticed that extracts from the leaves, stems and retted fibre inhibit the growth of S.pyogenes and S. aureus. In addition the inhibition of the growth of S. pyogenes was more than that of S. aureus and the antibacterial properties of S. rhombifolia are more concentrated in the stem than other parts of plant. Results of phytochemical screening also revealed abundant presence of saponins, glycosides and moderate presence of steroid in sidarhombifolia plant. The antibacterial property in the plant extract was due to the presence of various plant phytochemical compounds which were synthesized by plant parts in response to bacteria

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