A descriptive study to assess the knowledge regarding organ transplantation among staff nurses in a selected hospital at Mangalore. According to Indian government statistics, every year more than 10 lakh people in India are estimated to suffer from organ failure. Medical advancement and technology has demonstrated most miraculous achievement in modern medicine on organ transplantation. Nurses are expected to come forward and motivate the public about Organ transplantation. Nurses are the person who works with the client and family for 24 hours and so able to change the attitude of the public about the organ transplantation. Hence, the researcher felt to study the knowledge of staff nurses regarding organ transplantation. It may helps the patients to lead and achieve a normal life. The nurses were selected through non probability convenient sampling technique. The data was collected by administering the questionnaire to the samples. The result of the study showed that majority (68%) of staff nurses were having knowledge on organ transplantation. Nearly 32 % of nurses were need to update their knowledge on surgical procedure, preservation techniques and management regarding organ transplantation. The study result suggested that in service educational programme need to be planned on regular basis about organ transplantation.