Study of callus induction and regeneration of papaver somniferum l.

Papaversomniferum (opium poppy) is an herbaceous, annual and diploid plant that is very important because of pharmacological and strategic view in the world. The limitation of this plant farming, hard and law amount extraction of this plant alkaloids cause studying about tissue culture of papaver somniferum L. for getting high level of these components. This plant has a special group of alkaloids named benzylisoquinoline is due to secondary metabolites such as morphine, codeine and papaverineis a great value in pharmacy. In this research tissue culture of this plant to achieve the best hormonal levels for callus induction and regeneration examined from seed way culture. Small samples of leaves, roots and hypocotyls of 30-45 days from seedling s were transferred to different media with hormonal treatments. Best of hormone treatment for callus induction is 2mg/l 2, 4-D and 0.25mg/l BAP, 4mg/l 2, 4-D and 0.5mg/l BAP and best hormonal levels for regeneration includes 1mg/l BAP and 0.1 mg/l 2, 4-D.