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A Study on Methicillin and Vancomycin resistant Staphylococcus aureus from tertiary care hospitals, in Vidharbha region, India

Author: 
Wadhai, V. S. and Ashtankar, A. A.
Subject Area: 
Health Sciences
Abstract: 

Multidrug resistant methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community acquired infections and is on the rise. The glycopeptides vancomycin has been proposed as the drug of choice for treating such infections. The present study aimed at identifying the methicillin and vancomycin resistance staphylococcus aureus from tertiary care hospitals in Vidarbha region, (M/S) India. This study presents the report of methicillin and vancomycin heteroresistance in Staphylococcus aureus isolate from clinical samples. The original isolate was resistant in vitro to methicillin and fewer to vancomycin. Resistance confirmed for all isolates with E-tests using strips of methicillin MIC of >265 mcg/ml and vancomycin MIC of >256 mcg/ml. MRSA were isolated and identified from different clinical samples using conventional methods. Antibiogram of the isolates and MIC were determined following CLSI guidelines. All Multi Drug resistant Staphylococcus aureus isolates were MRSA. Only four Vancomycin resistant Staphylococcus aureus were found, out of the all MRSA isolates. All MRSA had a Methicillin MIC > 265 mcg/ ml except one showing 5.0 mcg/ml that of three VRSA had a Vancomycin MIC > 265 mcg/ ml and one shows MIC 24 mcg/ml.

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