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A sustainable technique of rapid multiplication of gerbera (Gerbera jamisonii L.) using in vitro seed culture

Author: 
Islam, M. M., Mamun, A. A., Dash, P. K., Kundu, R. R. and Akter, J.
Subject Area: 
Life Sciences
Abstract: 

The Gerbera production continues to experience propagation problem from division of clumps. Traditional approaches of propagation through clumps have wide range of limitation. In order to address the problem of propagation technique in a system designed to develop a sustainable protocol using in vitro seed culture was assessed. The goal of the present research is to develop a viable protocol for rapid propagation of Gerbera. The specific objective was to select a superior genotype responding to mass in vitro propagation by using seed explant. Sterilized seeds of nine gerbera genotypes were germinated on MS medium supplemented with BAP 3.0 mgL-1. In vitro grown seedlings after 30 days of seed culture were transferred onto MS medium containing BAP 2.0 mgL-1 + NAA 1.0 mgL-1 to allow multiple shoot formation. Elongated shoots were rooted on MS medium supplemented with IBA 1.0 mgL-1 for rooting. The regenerated plantlets were transferred to field after hardening under controlled environment. All the steps of this propagation system i.e. seed germination, multiple shoot differentiation, rooting and hardening was highly genotype dependent. Results indicate that genotype ‘Kristen’ showed highest germination frequency and number of leaves plant-1, Aladin exhibited the higher shoot regeneration, Silvester as well as Wink genotype showed greater response in in vitro shoot elongation. Pepe genotype showed maximum root formation and root elongation. Maximum survivability rate was found with the genotype Kristen while lowest was recorded in Sonata. Kristen, Aladin and Alabanda may be the suitable gerbera plant types for in vitro propagation through seed explant.

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