A study was conducted by decomposing of Parthenium hysterophorus (Linn.) weed plants with farm yard waste; maize waste and chick waste in the ratio3:4:2:1 which allowed on an anaerobic fermentation fraction of the C-containing organic colloids of nanoparticles and organic compounds. The carbon containing nanoparticles of humic substancesarehumic acid, Fulvic acid and organic compound content was in the form of GA3, IAA and BAP amount of to 0.13 %, 0.002%, and 0.04% per unit respectively. Further, the above nano particles were subjected to FT-IR, HPTLC and HPLC and crystallization of crude plant growth hormones for confirmation.