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In vitro callogenesis from the mature explants of croton scabiosus bedd. (euphorbiaceae)

Author: 
Sugali Salamma and Boyina Ravi Prasad Rao
Subject Area: 
Life Sciences
Abstract: 

Croton scabiosus Bedd. (Euphorbiaceae), an endemic tree of southern Andhra Pradesh, India is known to represent a small population and found primarily affected by recurrent fires and secondarily by seed pathology and poor germination in the natural habitat warranting immediate conservation attempts. With an objective to propagate the species in vitro through somatic embryogenesis, an attempt has been made to develop a protocol for callogenesis by using different explants collected from natural population. The explants were cultured on different media (MS, B5, and WPM) containing 2% sucrose, 0.8% agar-agar and different concentrations of plant growth regulators. After sterilization callus induction was tested with leaves, internodes and petioles using 2, 4-D, 2, 4 5-T, DICAMBA and NAA in different concentrations. All the plant growth regulators except NAA are found to induce callus proliferation. The maximum callus proliferation by inter nodes was observed on MS medium supplemented with DICAMBA 0.5 mg/l. This is the first successful attempt to establish consistent callus formation from inter nodal segments of Croton scabiosus and this procedure can be developed as an in vitro regeneration protocol for multiplication of plantlets.

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