Whether storage facilities alter HbA1c values?

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Author: 
Malligai, E., Divya Sri, L.R., Murugavel, S.K. and Manikandan, N.
Subject Area: 
Life Sciences
Abstract: 

Hemoglobin A1C (HbA1c) has been recognized as a diagnostic marker for diabetes mellitus by the ADA in 2013. Accuracy is extremely important to diagnose the disease. In case of HbA1c measurements, accuracy may be affected by various factors like transportation, storing facility and temperature alterations etc. Therefore, we designed this study to find out whether temperature and storage timings alter HbA1c results. Aım: The aim of the study was to find the comparison of HbA1c levels at different time intervals and storage temperatures. Materıals and Methodology: Paired Samples were collected from subjects attending master health check-up program irrespective of their diabetic status. In the first step, samples were processed immediately and the values were noted down. Then the first set was stored at 24oC and re -estimated after 8 hours and 16 hours. The second set was kept at 4oC and estimated after 8 hours and 16 hours. 18 samples alone from second set (4oC) were measured at 30 hours. All the samples were measured using BIO-RAD D10 machine based on HPLC ion exchange chromatography. Results and Conclusıon: Statistical analyses were done using Paired student t test and Pearson correlation coefficient in SPSS software version 21. Linear regression analysis was used to visually represent the difference. Our study did not show any major difference in HbA1c values irrespective of temperature and duration of the storage.

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