In vitro establishment of Wolbachia infection in the ovarian cell lines of (DZNU Bm-12) silkworm, Bombyx mori L

The Wolbachia is a maternally inherited, gram negative, obligate, intracellular proteobacteria which is found in number of invertebrates, except in the silkworm. To understand the Wolbachia-hosts interaction and molecular mechanism by which Wolbachia would interfere with viral replication,This symbiotic Wolbachia bacterium was isolated from RML-12 Aedes albopictus cells and was transfected into silkworm ovarian cell lines (DZNU Bm-12) maintained under in vitro standard conditions. The infection level was determined by PCR assay. None of the 1 to 40th passage showed infection in the silkworm. However, the techniques helped in understanding the incompatibles involved in non-infection of Wolbachia in silkworm. This study has opened out the scope for further assessment on the compatible factors.