Assessment of genetic variation among different accessions of oat (avena sativa l.) using rapd markers

The present study was carried out to assess the genetic variation in oats (Avena sativa L.) at DNA level. The three decamer primers, RAPD primers (GLD-18, GLE-08 and GLA-03) were used to estimate genetic diversity in Avena sativa genotypes. The assay revealed a large amount (65%) of polymorphism. The total of 20 bands were scored for three RAPD primers ranging from 4-9 corresponding to an average of 4.2 bands per primer and 13 bands of these were polymorphic. Polymorphic bands for each primer ranged from 0-100%. One primer out of three generated a total of 9 bands of which 9 (100%) were scored as polymorphic. Similarity matrix reveals the maximum similarity between varieties JHO 99-1 (S1) with JHO 822 (S3) and JHO 851 (S4) with JHO 2000-4 (S5) (Similarity Indices 0.880 and 0.903 respectively). While distantly related varieties were JHO 99-2 (S2) and JHO 99-1 (S1) (similarity indices 0.609). The RAPD cluster showed two major clusters namely cluster-I and cluster-II comprising of two varieties each. Cluster-I includes two cultivars namely JHO 99-1 (S1) and JHO 822 (S3). The major cluster-II includes JHO 851 (S4) and JHO 2000-4 (S5). The cultivar JHO 99-2 (S2) occupies a distinct place as revealed in the dendogram constructed. In present investigation RAPD markers revealed high degree of polymorphism (100%) among the five accessions of Avena varieties using GLE-08.