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A comparative study of estimation of urinary protein by turbidimetric methods vs pyrogallol red molybdate method

Author: 
Harmanjot Kaur, Dr. Gitanjali Goyal and Jagroop Singh
Subject Area: 
Life Sciences
Abstract: 

Background: The Concentration of total proteins in urine is a good index of renal function, but its determination is found to be unreliable. The pyrogallol red molybdate (PRM) method for urine total proteins is being widely used in most of hospitals because of its high sensitivity, better precision and its practicabilty. Material and Methods: The cross sectional study was conducted in department of biochemistry in the collaboration with department medicine in Guru Gobind Singh Medical College and Hospital, Faridkot. Out of 60 patients, 31 were males and 29 were females. Result: A statistically significant correlation was found between Pyrogallol red molybdate with Turbidimetric methods was highly significant with p value of ≤0.0001 and r value of 0.68, 0.86, 0.97 respectively. Conclusion: From this study, it was concluded that turbidimetric methods are correlated with pyrogallol red molybdate but there is significant difference in mean. So, pyrogallol red molybdate is method of choice for detecting proteinuria

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