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Analysing fatty acids content in winged bean (p. tetragonolobus (l) dc) through half seed chromatography

Author: 
Nurtjahjo D Sasongko, Siti Samiarsih and Nettyani Naipospos
Subject Area: 
Life Sciences
Abstract: 

Indonesia, the second largest mega diversity in the world. It has a wide array of plants species including Winged bean (Psophocarpus tetragonolobus (L) DC). Here, this crop is cultivated in many areas though they are not consumed daily leads to very limited effort to develop it. Developing this crop may attract people to cultivate and so consumption like as source for industrial oil. Selections for high fatty acids content seeds, so far are done by selecting good quality of seedlings through selection of the whole seeds. Current study initiated the use of half seed analysis on gas chromatography (GC) to collect data of high fatty acids content of winged bean. Six genotypes were collected from Sumatera (SMT 1 and 2), Java (1 and 2), Bali and West Nusa Tenggara (WNT), and used as the research material. The SMT genotypes are long pod genotypes (27 and 20,5 cm), Java have either long or short genotypes (27 cm length and 10.3 cm). Bali and WNT genotypes were not clear as they were collected as seeds instead of pods. The long pod genotype has total 8-22 seeds but only 6-8 seeds for short type. The SMT seeds germinated 100% but other genotypes showed lower rate of germination (65% and 55%). of those fatty acids, the Sumateran seeds contain40-50% palmitic acids but only few numbers of seeds from other genotypes. Three individuals were high in palmitic and oleic acids content. The high palmitic acid content reflected the present of putative genes with with four or 3 dominant alleles (PPPP, PPPp) in few individuals. This data showed if half seed was able to be used as source for GC analysis.

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