Background: The ability to respond quickly and effectively to a cardiac arrest situation rests on nurses being competent, prepared and up-to-date in the emergency life-saving procedure of cardiopulmonary resuscitation. The aim of this study was to determine the effect of educational training of cardiopulmonary resuscitation on nurse's practices regarding cardiopulmonary resuscitation in pediatric intensive care unit and Emergency Department. Materials and Methods: a quasi-experimental research design was used. All nurses who work in the Pediatric Intensive Care Unit and Emergency Department in El- Mobarra hospital were included in this study (60 nurses). Structure interview sheet was used to assess nurse's knowledge before and immediately after the educational training program about CPR. Practical test sheet was used to assess nurses' practic before and immediately after the educational training program about CPR. The Results of the study revealed that before educational training program the total scores of nurse's knowledge were good, fair and poor with percentage 15 %, 6.7% and 78.3% respectively. Whereas, after educational training the total score of knowledge for all studied nurses (100%) was good. All nurses' practice (100%) was poor before educational training. While after educational training 60%, 33.3% and 6.7 of nurses' practice were good, fair and poor respectively. Conclusion: The mean knowledge scores of nurses increased immediately after implementation of the program with a significant statistical difference. As well, the mean practice scores of the study group subjects were higher immediately after the implementation of the program with a high significant statistical difference. In addition, a positive correlation was found between knowledge and practice scores of the study subjects. Continuous In–service training programs for updating the knowledge and practice of nurses working with cardiac arrest pediatric patients about CPR was recommended.