Effect of pretreatment for delignification and sugar recovery for bioethanol production from arecanut husk waste

To meet the challenges of generating sufficient and sustainable energy, a potentially viable alternative is to use cellulosic biomass for second-generation biofuel and bioenergy production. In the present study, Arecanut husk is cheap abundant lignocellulosic raw material in the agricultural waste; it is available as alternative feedstock for bioethanol production. The moisture content of the fresh raw material (68.39 ± 0.20 %). Arecanut husk raw material were collected and dried in the hot air oven at 800 C for 48hours. The dried arecanut husk raw materials were subjected by powdered sequentially using hammer mill, ball mill and flour mill to obtain fine sized particles. The particles were separated by using a range of graded sews. Then sieved the different particle size such as sample-A (0.28 ± 0.01 mm), sample-B (0.43 ± 0.02 mm), and sample-C (0.64 ± 0.01 mm). The chemical analysis of arecanut husk raw material was done by using pretreatment methods. In acid hydrolysis the more amount of reducing sugar was found in the sample-A (4.12 ± 0.02 mg/g), non-reducing sugar in the sample-B (0.37 ± 0.01 mg/g), Protein in the sample-C (5.95 ± 0.06 mg/g) and Carbohydrate in the sample-B (2.82 ± 0.01 mg/g) of Arecanut fiber. In alkaline hydrolysis the more amount of reducing sugar was found in the sample-A (1.6 ± 0.10 mg/g), non-reducing sugar in the sample-A (1.02 ± 0.03 mg/g), Protein in the sample-A (7.40 ± 0.15 mg/g) and Carbohydrate in the sample-B (2.43 ± 0.02 mg/g) of Arecanut fiber.