Flow injection spectrophotometric determination of trifluoperazine hydrochloride using oxidative coupling reaction

A flow-injection (FI) spectrophotometric procedure is proposed for Trifluoperazine Hydrochloride (TFPH) determination in pharmaceuticals. The proposed method is based on the injection of 50 µL sample solution into an oxidizing agent stream of ammonium ceric salphate with the optimum flow rate of 2.0 ml min-1. The violet [TFPH- sulphanilic acid (SA)] complex is monitored at 545 nm. The (FI) system and the experimental conditions were optimized. Under the optimum conditions, calibration graph was obtained for 0.5-120 µg ml-1and the detection limit 0.0459 µg ml-1. The correlation coefficient was 0.9990. The method was successfully applied to the determination of this drug in pharmaceutical formulations with a sample throughput of 120h-1. The results obtained by the proposed method were in good agreement with those obtained by the official method at 95% confidence level.