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Gc-ms based phytochemical analysis and antimicrobial screening of non-polar fraction of withania coagulans

Author: 
Syed Tariq Ali, Syed Kashif Ali, Muneera Naz Baloch, Abdul Wahab and Syed Abdus Subhan
Subject Area: 
Physical Sciences and Engineering
Abstract: 

Withania coagulans, a medicinal plant traditionally use for the treatment of a variety y of human ailments particularly in diabetic. Investigation of the phytochemical composition and antimicrobial activity of non-polar fraction of ethanol extract of Withania coagulant twigs is discuss in present report. The phytochemical constituents of non-polar fraction of ethanol extract of twigs were studied through gas chromatography-mass spectrometry (GC/GCMS). A total of eleven compounds were identified which include sterols, fatty acid and their methyl esters. The Kovat’s Retention Indices were also used to support the identification of these compounds. Significant antimicrobial activity of the n-hexane fraction has been observed against several bacterial strains. The distinctive chemical composition of W. coagulans twigs along with substantial antimicrobial activity not only authenticate their traditional medicinal uses but also indicate their potential as a source of natural antimicrobial compounds.

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