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A Phytochemical study of two formulations of Aloe barbadensis Miller to support their pharmacotherapeutic difference as per clinical study w.s.r. dysmenorrhoea

Author: 
Dhiman Sonia, Kumar Ajay and Dhiman Monika
Subject Area: 
Health Sciences
Abstract: 

Aloe barbadensis Miller showed significant results in management of dysmenorrhoea in the form of Musabbar i.e. 25.11% more relief than Swarasa, significant at p<0.05. (t =3.283). To evaluate the reason of pharmacotherapeutic difference, analytical study was done to analyse phytochemicals in both formulations. Kumari contain properties as: - Guru, Snigdha, Pichhial Guna; Tikta, Madhur Rasa; Sheeta Veerya whereas Musabbar has: - Laghu Ruksha, Teekshna Guna; Katu Rasa; Ushna Veerya. Swarasa was exuded from pulp and sap of leaves. Musabbar was prepared by heating sap at low temperature up to complete evaporation of water. Qualitative tests, T.L.C. and UV spectral analysis were done on different extracts of both formulations to assess phytochemicals. Drug was curacoa aloe, anthraquinones, cardiac glycosides, protein; sapponins were present in both formulations. Phytosterols were present in juice not in Musabbar, Polysaccharides were present only in juice but monosaccharides and disaccharides were present in Musabbar, Flavanoides were present in both extracts of Musabbar, T.L.C. suggested presence of aloin, polyphenols, cholesterols, proteins and glycosides in Musabbar. Spectrum of acetyl groups and Phenolic compound were observed in UV spectral analysis of both formulations. Musabbar contains more anthraquinones having similar chemical structure to prostaglandin substrates to act as false substrate thus blocking prostaglandin synthesis, Musabbar contains glycosides, monosaccharide and disaccharides but Kumari Swarasa had only polysaccharides. So better result of Musabbar, may be due to these chemical constituents. Phytochemical study showed that drug prepared was genuine and difference in phytochemicals and Rasapanchaka supports the difference of results in clinical study.

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