Rapid diagnostic method by sediment cytology for identification of oral neoplasms by residual cells

Aim: To determine the diagnostic value of sediment cytology in early diagnosis of oral neoplasms. Material and Methods: Twenty oral biopsy specimens diagnosed of Oral squamous cell carcinomas were received in 10% formalin fixative willbe analyzed for the cytological study. The material required for the study is left over formalinfrom specimen bottles in which the biopsies are received. Cytological smears are prepared by centrifuging the fixatives in which the biopsy specimens are received and stained with modified Papanicolau stain (PAP). All specimens were analyzed for malignancyand intensity of expression were compared. Statistical analysis was done using Chi Square test to compare the intensity of expression in different study parameters based on the cellularity in malignant cases. Results: All the 20 cases were adequately cellular and other cytological parameters were altered with increase in cellularity showing malignancy. On comparison of intensity of expression of nuclear pleomorphism was 6.190 withP-Valueof 0.04, cellular pleomorphism was 6.190 with P-Valueof 0.04and hyperchromatic nucleiwas19.333 with a P-value of 0.001which was statistically significant. Conclusion: Our study showed all cases were malignant with increase in cellularity allcytological parameters were altered. On comparing the intensity of expression of nuclear pleomorphism, cellular pleomorphism and hyperchromatic nuclei showed statistical differences. In laboratory for preliminary diagnosis of oral biopsies sediment cytology can be an alternate methodology in histopathology