
Biofertilizers are the substance that contains microorganism's living or latent cells. bio fertilizers increases the nutrients of host plants when applied to their seeds, plant surface or soil by colonizing the rhizosphere of the plant. The present research work was comprises to valuable medicinal plant of Andrographispaniculata for morphological, biochemical, and enzyme contents of various stages of its growth. The inoculants such as Azotobacterchroococcum, Ppseudomonasfluorescens, and vermicompost are singly and in combinations are allowed to grow in similar environmental conditions. the morphological parameters such as shoot length, root length, leaf length, leaf width, total leaf area, shoot girth, number of branches per plant, plant height were observed. The biochemical namely chlorophyll, “a” chlorophyll “b” and total chlorophyll, and carotewnoids were measured. The enzymes such as catalase, and paroxidase were observed in 30, 60, and 90 days after sowing in Andrographispaniculata plants. The maximum growth was measured in combined application of Azotobacterchroococcum, Ppseudomonasfluorescens, and Vermicompost alone. The minimum growth was measured in control.