Copper (II) complexes containing mixed ligands, Schiff bases (L1=(2-carboxyphenyl)-pyridine-2-ylethyleneamine; L2=5-Br-(2-carboxyphenyl)-pyridine-2-ylethyleneamine and L3=5-NO2-(2-carboxyphenyl)-pyridine-2-ylethyleneamine) and 1, 10-phenanthroline has been synthesized by conventional methods. These copper (II) complexes has been characterized by elemental analysis, molar conductance, magnetic moment, FT-IR, electronic spectra, ESR spectra, electrochemical analysis, flourescence spectra and thermal analysis. On the basis of these spectral studies, it is revealed that all the complexes exhibited 1:1 (metal:ligand) ratio with the coordination number 6. The antimicrobial analysis showed that copper (II) complexes containing mixed ligands, Schiff bases (L1, L2 and L3) and 1, 10-phenanthroline is more active than only ligands.