Objective: The aim of the present study was to standardize Payapro™ Premix by quantitative estimation of apigenin and glycyrrhizin by reverse phase high performance liquid chromatography (RP-HPLC) and high-performance thin layer chromatography (HPTLC) methods. Payapro™ Premix is a polyherbal formulation used as galactogogue containing seven herbs including Cuminum cyminum and Glycyrrhiza glabra. Materials and methods: RP-HPLC and HPTLC methods were developed for the standardization of Payapro™ Premix by quantitative estimation of apigenin and glycyrrhizin, the bio-active constituents of Cuminum cyminum and Glycyrrhiza glabra respectively. The developed methods were validated on various parameters, including linearity, precision, accuracy, LOD, and LOQ as per ICH guidelines. Results: The RP-HPLC and HPTLC analysis methods were selective for the polyherbal formulation. Both the methods had specific linearity range with regression coefficient ≥ 0.995. The apigenin and glycyrrhizin estimation methods were precise (% RSD 0.7 and 0.4), accurate (average recovery values were 96.28 % and 95.83 %), LOD (sensitive) (0.0054 µg/mL & 0.016 µg spot-1), LOQ (reliable) (0.0162 µg/mL & 0.048 µg spot-1), respectively. Methanolic extracts of polyherbal formulation showed the presence of significant amount of apigenin (50 µg/g) and glycyrrhizin (1200 µg/g) using developed methods. The comparison of different batches for these markers was found to be uniform which ensures their quality. Conclusion: The present work emphasized on standardization of the polyherbal formulation by determination of bio-active marker constituents. The developed methods can be used to standardize other samples containing Cuminum cyminum and Glycyrrhiza glabra.
IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.
