The study on socio-economic determinants of exclusive breastfeeding on working mothers in AMAC was carried out to find out if variables like mothers level of knowledge, attitude and other socioeconomic variable affect the practice of exclusive breastfeeding practice. The respondents were selected through simple random sampling. In all a total of 324 were sample for the study. Questionnaires, indebth-oral interview and focus group discussion were the instruments used for data collection. Data was collected randomly from various immunization clinics in AMAC, and analyzed using simple percentages and chi-square, testing methods. The findings showed that the variables like mothers level of knowledge, mother's attitude towards exclusive breastfeeding, mothers, level of education among others were all significant to their practice of exclusive o breastfeeding, while spouse support and cultural beliefs are not associated with exclusive breastfeeding practice. In conclusion, the study recommended for better practice of exclusive breastfeeding. More education for mother's and the general public on the benefits and need for exclusive breastfeeding. Also the extension of maternity leave to enable other's have enough time to practice exclusive breastfeeding, and provision of creches in all offices to enable working mothers breastfeed their babies properly and teaching of mothers ways of expressing of o breast milk and storage of expressed breast milk so as to improve the rate of exclusive breastfeeding practice by working mothers and the society at large.