Determination of optimal conditions to extract antibacterial substances from Prunusarmeniace seeds

The present study aims to determine the optimum extraction conditions for Prunus armeniace seeds and test their effectiveness against a group of pathogenic bacteria. Five solvents used are acetone, ethanol, methanol, ethyl acetate and disitilled water to obtain of crude extract of Prunus armeniace seeds, which tested the effectiveness of antibacteria on five types of bacteria are Pseudomonas eroginosa, Pseudomonas oryzihabita, Proteus varaplis, Klebsila pneumonia and Staphylococcus aureus to determine the most efficient solvent extraction of them, and then was use of a series of concentrations of the solvent is more efficient 0, 20, 40, 60, 80 and 100% to determine the most efficient concentration of solvent optimization, and then was determined minimum inhibitory concentration(MIC) of the extract more efficient. The results of the current study showed that the most efficient in the extraction solvent is ethanol that they were the diameters of inhibition zone are 13, 15, 16.66, 16.33 and 19.33 mm for the types of bacteria above, respectively. The results showed that the concentration of ethanol was 100% in the optimal inhibition of bacteria, amounting to diameters of inhibition zone at this concentration of 30, 31.66, 12.16, 24.16 and 30.66 mm for each of the bacterial species above, respectively. The results also found that MIC of Pseudomonas eroginosa, Pseudomonas oryzihabita and Proteus varaplis is 23 mg/ml, but MIC of Klebsila pneumonia is 16 mg/ml, while the MIC of Staphylococcus aureus is 13 mg/ml. The optimal conditions for extracting the most effective composites against pathogenic bacteria from Prunus armeniace seeds are using ethanol solvent with concentration of 100%.