This study focused upon the seasonal variation of physico-chemical parameters in Rockfort Thayumanavar temple pond, Samayapuram Mariaman temple pond, Gunaseelam Prasanna vengadajalapathy temple pond and Vayalur Murugan temple pond water. Water quality is assessed during September 2012 – September 2013 using standard procedures. Water samples were assessed by analyzing various physico-chemical parameters such Turbidity, Electrical Conductivity, Total Dissolved Solids (TDS), Tolal alkalinity, Magnesium, Chloride and Fluoride of the temple ponds water in Tiruchirappalli district. The highest value of the physic-chemical characteristics such as (EC – 2230 μmhos/cm, TDS – 1561mg/l, CaCO3 – 516, Mg – 48 mg/l, Cl – 380 mg/l) present in Rockfort temple pond. The lowest value of EC – 515 μmhos/cm, TDS – 361mg/l, Cl – 64 mg/l present in Gunaseelam temple pond and CaCO3 – 116, Mg –3mg/l present in Vayalur Murugan temple pond. There is no change in fluoride value (0.2mg/l) in every month of all the four temple ponds during the study period. Thus the present study reveals that the water of the temple ponds can be polluted moderately and unfit for human consumption. It needs sufficient treatment and management.antibacterial activity against multidrug resistant Staphylococcus aureus- MRSA and VRSA.