Antibiotics are frequently used in clinical practice. The antibiotic-resistant bacteria due to unthoughtful and indiscriminate use of antibiotic is a major issue which is affecting healthcare delivery throughout the world. Available literature highlights the necessity of rationalization of antimicrobial therapy in developing countries. Study tool: A cross sectional questionnaire based study was conducted after the approval from Institutional ethics committee over the time of one month at Acharya Vinoba Bhave Rural Hospital (AVBRH) Sawangi (Meghe) Wardha. A structured questionnaire was prepared and validated by the experts, which consisted of three parts. They included questions regarding knowledge, attitude and practice of post graduate students about antibiotic use and resistance prescribed in there hospital. Study population: Post graduate students of all three years working in AVBRH. Study procedure: Each post graduate student was explained the objectives of the study and their willingness to participate in the study were obtained. After the briefing, questionnaire was distributed and the students were asked to respond to the questions completely and anonymously. Completed responses were collected for analysis. Conclusion: Medical education should include strategies to change the attitude and practices apart from raising knowledge for improved patient outcomes. Medical professionals should be tailored with a sense of responsibility that, as prescribers, their responsibility pertains not only to the patients benefit and wellbeing but also to the society at large.