This multicultural twin study examined the structure and sources of individual differences in agreeableness. This study assessed cultural influences on the agreeableness component of the personality. A pair of 90 twins in the age group of 3-6 years from five cultural zones of state Haryana was taken. The data were analyzed for agreeableness culture wise and yield new insight into the trait. The results revealed that there was a significant cultural difference in agreeableness trait of personality in monozygotic twins. The respondents of Ahirwal zone had better personality traits against their counterparts.