Plant extracts have a long history of applications as antimicrobial agents, but their use as wood preservatives has rarely been reported. This study deals with the antifungal activity of the Lawsonia inermis extracts against four cellulolytic wood fungi, in order to find new bioactive natural products. The methodology used is based on a measuring the inhibition halos produced by discs impregnated with the extracts and establishing their Minimum Inhibitory Concentrations (MIC). The extracts, object of the survey, showed a strong antifungal activity of ethanolic extracts of leaves against all tested fungi. These results suggest the possible exploitation of the Lawsonia extracts as potential botanical effects in ecofriendly control of wood biodeterioration by fungi.