Background: Microorganisms like bacteria, viruses are common water contaminants and they lead to waterborne disease and epidemics. The aim of this study was to assess the quality of pond water. In this respect we evaluate Coliforms and other bacteria present in the pond water environment. Six samples of pond water were collected from six major ponds of the Patna urban area which is used for pissiculture. Methods: Samples were processed in isolation of different microbes by using Mac Conkey agar, EMB agar and etc. Microbes were identified by different biochemical test related to bacterial metabolism. All the experimental work was carried out between the periods of December, 2017 to February, 2018 at the Department of Biotechnology, A. N. College, Patna. Results: The result indicates the all the six ponds contain Coliforms for above the APHA limits. They also show the presence of Pseudomonas aeruginosa, Klebsiella, Shizella, Enterobactor etc., which could further endanger for both fishes and consumers. Conclusion: Results suggest that of monitoring the water quality from time to time. It will help to highlight the route of contamination.