Comparative study of organophosphate pesticide (methyl parathion) effects on protein profile in brain tissue of heteropneustes fossilis and channa punctatus

The present study focuses on the comparative evaluation of protein profile responses in the brain tissue of Heteropneustes fossilis and Channa punctatus exposed to the organophosphate pesticide, methyl parathion. Specimens of both species were collected from local ponds located approximately 15 km away from Kakatiya University, Warangal. The protein profiles of brain tissues from control and pesticide-exposed fish were analyzed using the SDS-PAGE technique. In control conditions, the brain tissues of both H. fossilis and C. punctatus exhibited eight distinct protein bands. However, exposure to methyl parathion induced noticeable alterations in these protein bands, including changes in band intensity and the appearance of new or antagonistic protein bands, which varied between the two fish species.