The current research investigates Comparison of inner to outer leaflet and outer leaflet density of arenaviruses. The results indicates that to understand the density of vesicles as compare to the arenaviruses, and the ratio of various vesicles was taken and plotted the transect plots. Here we are trying to make an assay to detect that how much protein is inserted in the membrane so I have looked at the vesicles from lots of different sources and groups. It was concluded that ratio of inner to outer is constant in vesicles.