Aim: To assess the knowledge and practices regarding biomedical waste management among healthcare professionals. Background: Health care waste is a unique category of waste by the quality of its composition, source of generation, its hazardous nature and the need for appropriate protection during handling, treatment and disposal. Mismanagement of the waste affects not only the generators, operators but also the common people too. Methods: Descriptive study was conducted in different departments of multi specialty hospital. Conclusion: Knowledge level of the samples was good and excellent but some practice levels were not up to the standard. Corrective steps were taken to improve the practices.