The effect of amonium sulphate concentration on the isolation result of bromelin skin and Tubers of pineapples

Pineapple is widely known by Indonesia people because the plants are widely grown in tropical climates Indonesia. Pineapple is often used as meat diggers because it contains a component of bromelin enzyme which has a sulfhidril (-SH) protease that can hydrolyze peptide bonds in proteins into bundles of amino acid compounds are simpler so that it can make meat softner and help the process of digestion. The aim of this study was to isolate bromelin enzyme from pineapple tubers and pineapple skin which is pineapple waste with ammonium sulphate salt with four treatments (40%, 60%, 80%, and 100%) and three repetitions for having rendemen and activity enzyme. Calculated rendemenanalyzed by one way Anova. The most optimal ammonium sulfate concentration for isolate enzyme is at 60% concentration, with rendemen of 1.67% and enzyme activity of 3.33 U/mL. The concentration of 40% has a rendemen of 0.37% with enzyme activity of 6.67 U/mL, 80% concentration with 0.91% rendemen with 1.67 U / mL enzyme activity, and 100% concentration has 0.94% rendemen with activity enzyme 1.67 U/mL.