The present study was designed to determine the bioactive components in aqueous hot extract of Eugenia uniflora leaves. Phytochmical screening of the extract revealed the presence of various metabolites like alkaloids, flavanoids, cardiac glycosides, phenols, tannins etc., GC-MS analysis in aquous extract was carried out using Perkin Elmer GC Clarus 500 system comprising AOC-20i Auto-sampler and a Gas chromatography interfaced to a Mass spectrometer (GC-MS) equipped with a Elite-5MS (5% Diphenyl 95% Dimethyl Poly Siloxane) Fused capillary column (30× 0.25m 1D × 0.25m dF). GC-MS analysis has shown the presence of 39 biomolecules corresponding to 39 peaks. From the result it is evident that the aqupous leaf extract of Eugenia uniflora leaves contains various phytocomponents and is recommended as a plant of phyto pharmaceutical importance.