The scope of current research is to establish the impact of different fertilizers (ammonium nitrate, mono- and diammonium phosphate, liquid nitric fertilizer, urea and compound fertilizer NPK) produced by “Agropolychim” AD on chemical parameters and soil enzymatic activity related to common winter wheat and oilseed rape. Fertilizers are applied before sowing process. The experiment is repeated twice into greenery. Biogenic elements and enzymatic activity estimation is made after soil sampling and during wheat pick up phase and at the end of coleseed’s leaf rosette phase. Results show that the highest total nitrogen volumes in wheat samples are detected when fertilization with DAP is made, and in the cases with coleseed – the highest total nitrogen content is estimated when liquid nitrogen fertilizer UAN is applied. Total phosphorous content in both cases is highest when fertilization with DAP is done. Recorded content of total potassium in wheat and coleseed samples is highest in cases when compound fertilizer NPK is applied. It has been established higher content of these three macronutrient elements in case of wheat experiment. This fact proves coleseed’s larger needs of nutrient elements even in the early phase of culture development. Used fertilizers enhance the enzyme soil activity. The values for catalase and cellulase activity are higher with fertilized soils samples compared with control base case - soil sample without fertigation. Application of Ammonium nitrate and NPK increases catalase activity. In case of DAP application, in contrary, the activity of this enzyme is decreased as well as with wheat and coleseed. It has been estimated that cellulase activity is around 19% higher when fertilizers are applied compared with base case of non-treated soil with fertilizers. The best result for cellulase activity value is achieved with UAN application in both cases – wheat and coleseed. Tested chemical and enzymatic parameters can be used as specific chemical and biochemical markers for estimation of fertilized soils.