Aim: This study was aimed to assess the mothers’ oral health care knowledge and its impact on oral health status of their children. Methodology: 100 mothers along with their children in the age group of 3-5 years were included in the study. 17 point questionnaire was distributed to mothers to assess their knowledge regarding oral health care practices of their children. Oral examination of children was done and dmft score was recorded. Mothers were divided into two groups: group 1 whose children had dmft score <3 and group 2 whose children had dmft score >=3. Results: Very few mothers had knowledge about importance of deciduous teeth and oral hygiene practices. Significant difference (p<0.05) were found between group 1 and group 2 mothers’ knowledge about the role of prolonged breastfeeding and bottle feeding in causing tooth decay. 90% of mothers did not know about the role of fluoride in preventing dental decay. Conclusion: Children below 5 years spend most of their time with mothers. Mothers function as role model for their children. Therefore mothers’ knowledge, attitudes and beliefs regarding dental care need to be improved for better oral health status of their children.