Soil pH plays an important role in the survival of soil microbes. These effect the soil micro and macronutrients. Phosphorus (P) is a major growth macronutrient in the soil taken up by the plants for growth, development and yield of every crop. Phosphate solubilising bacteria (PSB) play role in phosphorus nutrition by enhancing its availability into rhizosphere by release from inorganic and organic bound sources in soil through phosphorus solubilisation and mineralisation. In the present study a comparative assessment has been made on the Pikovskaya agar medium (PVK) containing methyl red, methyl red orange and bromophenol blue. These are used for observing the differential phosphate solubilising activity of phosphate solubilising bacteria (PSB) in the potato growing soil. The study revealed higher number of PSB forms revealed in PVK containing methyl red followed simultaneously by PVK containing bromophenol blue and methyl red orange respectively.