Aim: To present a case with variation in the canal configuration and morphology of maxillary third molar, which was diagnosed and endodontically treated successfully. Knowledge about root canal anatomy and morphology of root canal system plays an important role in the prognosis of endodontic treatment and its success. There are many variations in number of roots and canal configuration in maxillary molars. Undetected anatomical variations of roots or root canals which remain untreated are one of the main reasons for endodontic failure. Moreover, treating the third molars present a great challenge for endodontists because of their known inaccessibility and variability. The present paper describes a case of a right maxillary third molar with a canal configuration rarely reported in the literature. The tooth had four different roots with four root canals, two individual palatal roots one mesiopalatal and one distopalatal.