NUTRIENTEVALUATION, PHYTOCHEMICAL ANALYSIS AND INVITRO MICROPROPAGATION IN NAVARA (ORYZASATIVA L.)

The study was conducted to assess the nutritional value, phytochemical compounds and invitro regeneration in Navara. The major nutrients in dehusked rice were Ca (10.3%), Fe (2.81%), Zn (2.78%), P (197%), VitB1 (0.77%), VitB2 (0.16%), VitB3 (1.26%), protein (8.8%), fat (2.4%) and carbohydrate (72.7%).GC-MS analysis of hexane extract of Navara revealed the presence of 113 phytochemical compounds, of which the major compounds were Cyclotrisiloxane hex methyl (52%), 5,7,9(11)-Androstatriene,3-hydroxy-17-oxo- (64%) and Olean-12-ene-3,16,21,22,28-pentol, 21-(2-methyl-2-butenoate), [3a,16a,21a(Z),22a]- (66%).To observe the callus induction and plant regeneration, different concentrations and combinations of growth regulators were added in MS medium. Better callus induction was observed on MS medium supplemented with 2,4-D 1mg/l, while MS medium supplemented with BAP1mg/l+NAA0.5mg/l induced the highest somatic embryogenesis. Better shoot multiplication was observed on MS medium containing BAP 0.5mg/l + IBA 1mg/l. Effective root regeneration was on MS + IBA 1mg/l. Present study provides a protocol to conserve and multiply this nearly extinct medicinal rice variety.