The study was conducted to assess the nutritional value, phytochemical compounds and invitro regeneration in Navara. The major nutrients in dehusked rice were Ca (10.3%), Fe (2.81%), Zn (2.78%), P (197%), VitB1 (0.77%), VitB2 (0.16%), VitB3 (1.26%), protein (8.8%), fat (2.4%) and carbohydrate (72.7%).GC-MS analysis of hexane extract of Navara revealed the presence of 113 phytochemical compounds, of which the major compounds were Cyclotrisiloxane hex methyl (52%), 5,7,9(11)-Androstatriene,3-hydroxy-17-oxo- (64%) and Olean-12-ene-3,16,21,22,28-pentol, 21-(2-methyl-2-butenoate), [3a,16a,21a(Z),22a]- (66%).To observe the callus induction and plant regeneration, different concentrations and combinations of growth regulators were added in MS medium. Better callus induction was observed on MS medium supplemented with 2,4-D 1mg/l, while MS medium supplemented with BAP1mg/l+NAA0.5mg/l induced the highest somatic embryogenesis. Better shoot multiplication was observed on MS medium containing BAP 0.5mg/l + IBA 1mg/l. Effective root regeneration was on MS + IBA 1mg/l. Present study provides a protocol to conserve and multiply this nearly extinct medicinal rice variety.