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Optimization of somatic embryogenesis induction in wild snake gourd (trichosanthes cucumerina l. var. cucumerina)

Author:

Devendra N. Kage, Vijaykumar, B. Malashetty and Seetharam, Y. N.

Subject Area:

Life Sciences

Abstract:

In this study, regeneration of Trichosanthes cucumerina L. var. cucumerina via somatic embryogenesis was investigated. Four different types of explant (Leaf, cotyledon, epicotyl and hypocotyl) from six-eight days old seedlings were used. Growth regulator treatments were one levels of 2,4-dichlorophenoxyacetic acid (0.3 mg/l) and three levels of 6-benzyl aminopurine (0.25, 0.5 and .75 mg/l). After seven weeks, cotyledon explants showed the highest potential in somatic embryo induction and the combination of 0.3 mg/l 2,4-D and 5 mg/l BAP had significant effect on somatic embryogenesis of Trichosanthes cucumerina L. var. cucumerina.

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