The aim of this work was evaluate the water potential, relative water content, and the osmoregulators in young plants of acapu submitted to water déficit. The experiment was conducted in a greenhouse at Federal Rural University of Amazônia, Belém, Brazil, in a period of 9 months. The experimental design utilized was completely randomized in factorial 2x4 (two water conditions: control and drought and four times of evaluation) with 5 repetitions, totaling 40 experimental units. The imposition of water deficit was obtained by suspension of irrigation in 30 days time, with first time (zero days of drought), time 2 (10 days), time 3 (20 days) and the time 4 (30 days). In these plants, was verified a reduction in water potential and RWC in the leaf, in the end of the experiment. The drought induced the increase in carbohydrate content, as well as in sucrose, proline and glycine betaine concentrations on leaves and also in the roots. The lack of water caused a reduction of starch in both organs studied. The accumulation of these osmorregulators in response to drought provided a decrease in water potential in these plants, reducing the effects of stress on the relative water content in the leaf.