Drosophila punjabiensis is as such common pest reported from India. Pesticides induce minor changes in the structure of reproductive organ of Drosophila, Here we examined the effect of Malathion and Chloropyrifos on Drosophila at 50% lethal concentration and control condition and influence results were obtained. Thus this study can be used for similar different common pests and induce effective pest management by using Drosophila as model organism.