Interspesific F1 hybrids between S. aethiopicum Acc.8971 and S. melongena var. DLP and Acc. 3305 were produced. Yet, recovered crosses were observed to have high percentage of flower abscission and fruit setting was never noted in them. Pollen tube study was conducted to unveil the pre-fertilization barriers causing hybrid sterility and breakdown. Fluorescence microscopy was used to determine pre-fertilization barriers. Pre-fertilization barrier in the form of inhibited pollen tube growth was noted.